Accurate Cultivar Authentication of Jujube Fruits Using Nano-Fluidic Genotyping of Single Nucleotide Polymorphism (SNP) Markers

Chinese jujube (Ziziphus jujuba Mill.) is an economically important fruit tree that highly adapted to marginal crop lands and can be grown under a very broad range of climate conditions. Jujube fruits produced from several traditional cultivars in Ningxia, China have enjoyed premium market price due their unique flavor quality attributes. One problem with the high-end adulteration cultivars. The accurate identification genetic identity single essential for cultivar traceability authentication. multi-locus SNP barcoding approach offers effective solution authentication fruits. To identify variant sequences nanofluidic array was used test efficacy this method DNA extracted pulp eight China. marker profiles identified each unambiguously based on multilocus matching ordination analysis. Results repeated sampling multiple same (using independent extractions) showed high level concordance, which demonstrated reliability genotyping platform. This handle 100 s 1000 samples per day (based platform used). It robust cost-effective has potential its practical application industry. developed protocol readily applied botanical other stone general.